ANTI GANGLIOSIDES MONOCLONAL ANTIBODIES AND THEIR USE IN THE SPECIFIC ACTIVE IMMUNOTHERAPY OF MALIGNANT TUMORS

• Main Authors: VALCARCEL, CARLOS M. A, LOPEZ, ANA M. V, ABRAHAM, AMPARO E. M, FERNANDEZ, ANGEL M. A, RODRIGUEZ, ROLANDO P, RUIZ, MARIA E. L
• Format: Patent
• Language: English; French
• Published: 20.07.1999
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

The present invention is related to the field of immunology, particularly the generation and selection of monoclonal antibodies (mAbs) against gangliosides (A bls) which antibodies are specific, have recurrent idiotypes and have value as immunoregulators of the immune response against those antigens, and to obtaining anti-idiotypic mAbs (Ab2s) generated by the immunization with the said anti ganglioside mAbs. Accordingly, an objective of this invention is to provide anti ganglioside mAbs (Abls) with idiotypes capable of inducing an antigen specific response (Abl ') in the absence of the antigen and the methods for its generation. Another objective of this invention is to provide anti-idiotypic mAbs (Ab2s) generated by immunization with anti ganglioside mAbs that have the above mention ed property and also capable of producing all Abl' response. A further objective of the invention comprises generating specific anti ganglioside mAbs (Abl, immunizing mice and other species with said antibodies an d selecting those antibodies capable of generating an antigen specific response (A bl'): generating anti-idiotypic mAbs by immunizing mice or other animal species with aforementioned anti ganglioside mAbs. It will be understood that where the term antibodies is used herein that fragments, derivatives and the like which have similar antigen grinding properti es are also intended. Many ways of arriving at derivatives and/or fragments are known i n the Art. Ways of producing derivatives and/or fragments include but are not limited to chemical cleavage and/or (re) assembly, recombinant production of single chai n antibodies and/or molecular recognition units (MRU's), CDR-grafting class switches, etc.