LIPOPEPTIDE DEACYLASE
Echinocandin B deacylase, a cell-associated enzyme from Actinoplanes utahensis, is purified to near homogeneity in a process comprising hydrophobic interaction chromatography, cation-exchange chromatography and gel filtrations. The enzyme is a heterodimer containing 18-KD and 63-KD subunits and is a...
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Main Authors | , |
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Format | Patent |
Language | English French |
Published |
29.05.2001
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Edition | 5 |
Subjects | |
Online Access | Get full text |
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Summary: | Echinocandin B deacylase, a cell-associated enzyme from Actinoplanes utahensis, is purified to near homogeneity in a process comprising hydrophobic interaction chromatography, cation-exchange chromatography and gel filtrations. The enzyme is a heterodimer containing 18-KD and 63-KD subunits and is a simple enzyme unaffected in activity by co-factors, metal chelators, or sulfhydryl reagents. The enzyme catalyzes the deacylation of the lipid aryl portion of lipid cyclicpeptide metabolites such as ECB and aculeacin. |
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Bibliography: | Application Number: CA19912043762 |