PROCESS FOR THE PRODUCTION OF HETERO-BISPECIFIC ANTIBODIES
For the production of hetero-bispecific monoclonal antibodies at least the genes for the light chain and for the variable part of the heavy chain are isolated from a hybridoma cell line which secretes an antibody with a desired specificity and they are inserted into a eukaryotic plasmid vector which...
Saved in:
Main Authors | , |
---|---|
Format | Patent |
Language | English French |
Published |
28.03.1995
|
Edition | 5 |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | For the production of hetero-bispecific monoclonal antibodies at least the genes for the light chain and for the variable part of the heavy chain are isolated from a hybridoma cell line which secretes an antibody with a desired specificity and they are inserted into a eukaryotic plasmid vector which contains a marker capable of selection together with a strong promoter, this expression vector is transfected into a hybridoma cell line which secretes antibodies with a second desired specificity, the cell line is cultured, the antibodies are obtained and the bispecific antibody is isolated. |
---|---|
Bibliography: | Application Number: CA19902012631 |