OVINE INHIBIN

OVINE INHIBIN A 34,500-dalton protein with inhibin activity is isolated from ram rete testis fluid using reverse-phase high-performance liquid chromatography and gel filtration. The isolated molecule is composed of two chains having molecular weights of about 18,000 and about 16,500 Daltons, which a...

Full description

Saved in:
Bibliographic Details
Main Authors RIVIER, JEAN EDOUARD FREDERIC, VALE, WYLIE WALKER, JR, SPIESS, JOACHIM, BARDIN, C. WAYNE
Format Patent
LanguageEnglish
French
Published 28.07.1992
Edition5
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:OVINE INHIBIN A 34,500-dalton protein with inhibin activity is isolated from ram rete testis fluid using reverse-phase high-performance liquid chromatography and gel filtration. The isolated molecule is composed of two chains having molecular weights of about 18,000 and about 16,500 Daltons, which are bound together by disulfide bonding and the longer of which is likely glycosylated. Microsequencing revealed the NH2-terminal portion of the 18kD chain to be . This 34.5kD protein specifically inhibits basal secretion of FSH, but not of LH, in a rat anterior pituitary monolayer culture system, exhibiting a half-maximal effective dose of 0.3 ng/ml. Furthermore, antibodies raised against a synthetic replicate of the N-terminal six residues of the 18kD chain are effective to reduce the activity of highly purified 34.5kD ovine inhibin and might be used to exert a profertility effect in rams, ewes and other mammals.
Bibliography:Application Number: CA19870540422