USE OF GYRASE INHIBITORS FOR DECONTAMINATION OF MYCOPLASMA-INFECTED CELL CULTURE

• Main Authors: ZEILER, HANS-JOACHIM, HUEBNER, GUENTER E, BRUNNER, HELMUT
• Format: Patent
• Language: English; French
• Published: 10.09.1991
• Edition: 5
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; ENZYMOLOGY; HETEROCYCLIC COMPOUNDS; HUMAN NECESSITIES; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Use of gyrase inhibitors for decontamination of mycoptplasma-infected cell cultures A method for decontaminating cell cultures comprising contacting cell cultures with quinolone- and 1, 8-naphthyridonecarhoxylic acids of the formula (I) in which R1 represents methyl, ethyl, propyl, isopropyl,cyclo propyl, vinyl, 2-hydroxyethyl,2-fluorethyl, methoxy, amino, methylamino, dimethylamino, ethylamino, phenyl, 4-fluorophenyl or 2,4-difluorophenyl, R2 represents hydrogen, alkyl having 1 to 4 carbon atoms, or (5-methyl-2-oxo-1,3-dioxol-4-yl)-methyl, R3 represents methyl or a cyclic amino group such as , in which R4 represents hydrogen, alkyl having 1 to 4 carbon atoms, 2-hydroxyethyl, allyl, propargyl, 2-oxopropyl 3-oxobutyl, phenacyl, formyl, CFCl2-S-, CFCl2-SO2-, CH3O-CO-5-, benzyl, 4-aminobenzyl or R5 represents hydrogen or methyl, R6 represents hydrogen, alkyl having 1 to 4 carbon atoms, phenyl or benzyloxomethyl, R7 represents hydrogen, amino, methylamino, ethylamino, aminomethyl, methylaminomethyl ethylaminomethyl, dimethylaminomethyl, hydroxyl or hydroxyethyl, R8 represents hydrogen, methyl, ethyl or chlorine X represents fluorine, chlorine or nitro and A represents N or C-R9, in which R9 represents hydrogen, halogen such as flourine or chlorine, methyl or nitro, or can also, together with R1, form a bridge of the structure -O-CH2-CH-CH3, -S-CH2-CH-CH3 or -CH2-CH2-CH-CH3.