ENHANCED LUMINESCENT OR LUMINOMETRIC ASSAY

An enhanced luminescent or luminometric assay, especially immunoassay, having a luminescent reaction between a peroxidase enzyme, an oxidant, a chemiluminescent 2,3-dihydro-1,4-phthalazinedione and a sensitivity enhancer of general formula I, < IMG > I The preferred materials are horse radish...

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Bibliographic Details
Main Authors WHITEHEAD, THOMAS P, THORPE, GARY H.G.H, KRICKA, LARRY J
Format Patent
LanguageEnglish
French
Published 27.01.1987
Edition4
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:An enhanced luminescent or luminometric assay, especially immunoassay, having a luminescent reaction between a peroxidase enzyme, an oxidant, a chemiluminescent 2,3-dihydro-1,4-phthalazinedione and a sensitivity enhancer of general formula I, < IMG > I The preferred materials are horse radish peroxidase as the enzyme, hydrogen peroxide as the oxidant, one of 4-iodophenol, 4-phenyl-phenol or 2-chloro-4-phenylphenol as the enhancer and either luminol or isoluminol as the phthalazinedione. In a particularly preferred embodiment the horse radish peroxidase is coupled to an antibody to the substance to be assayed and thereby acts as the assay label in an immunoassay.
Bibliography:Application Number: CA19840446973