MODIFICAÇÃO DA PRODUÇÃO DE GLICOPROTEÍNAS EM PLANTAS

• Main Authors: MARC-ANDRE D'AOUST, STEPHANIE AQUIN, LOIC FAYE, THOMAS PACCALET, CHRISTOPHE SOURROUILLE, VERONIQUE GOMORD, MURIEL BARDOR, LOUIS-PHILIPPE VEZINA, CAROLE BUREL, CHRISTOPHE RIHOUEY, ESTELLE MARQUET-BLOUIN, PATRICE LEROUGE
• Format: Patent
• Language: Portuguese
• Published: 30.09.2014
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNT1-GalT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNT1) fused to a catalytic domain of beta-1,4galactosyltransferase (GalT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.