"SUBSTRATO DE TOXINA DE BOTULINUM DE SOROTIPO A (BONT/A), BEM COMO MÉTODO DE DETERMINAR ATIVIDADE DE PROTEASE DE TOXINA CLOSTRIDIAL"

• Main Authors: ESTER FERNANDEZ-SALAS, LANCE E. STEWARD, KEI ROGER AOKI
• Format: Patent
• Language: Portuguese
• Published: 11.11.2014
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a lanthanide donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the lanthanide donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the lanthanide donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the lanthanide donor fluorophore and the acceptor.