processos para efetuar mutação no dna que codifica uma enzima de subtilase ou sua pré- ou pré-pró-enzima e para a manufatura de uma enzima de subtilase mutante

A subtilisin having improved storage stability or improved performance in detergents, wherein one or more amino acid residues situated in, or in the vicinity of a hydrophobic domain of the parent subtilase have been substituted for an amino acid residue more hydrophobic than the original residue, sa...

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Bibliographic Details
Main Authors L. N. SIERKSTRA, PETER MARKVARDSEN, CLAUS VON DER OSTEN, J. KLUGKIST, PETER BAUDITZ
Format Patent
LanguagePortuguese
Published 24.01.2012
Subjects
ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES
BEER
BIOCHEMISTRY
CANDLES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DETERGENT COMPOSITIONS
DETERGENTS
ENZYMOLOGY
FATTY ACIDS THEREFROM
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
RECOVERY OF GLYCEROL
RESIN SOAPS
SOAP OR SOAP-MAKING
SPIRITS
USE OF SINGLE SUBSTANCES AS DETERGENTS
VINEGAR
WINE
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Summary:A subtilisin having improved storage stability or improved performance in detergents, wherein one or more amino acid residues situated in, or in the vicinity of a hydrophobic domain of the parent subtilase have been substituted for an amino acid residue more hydrophobic than the original residue, said hydrophobic domain comprising the residues corresponding to residues P129, P131, I165, Y167, Y171 or BLS309 (in BASBPN numbering), and said residues in the vicinity thereof comprises residues corresponding to the residues E136, G159, S164, R170, A194, and G195 of BLS309 (in BASBPN numbering), with the exception of the R170M, R170I and R170Y variant of BLS309.
Bibliography:Application Number: BR19969608149