métodos e composições para detectar uma atividade biológica

• Main Authors: KURT J. HALVERSON, STEPHEN B. ROSCOE, JESSE D. MILLER, JASON W. BJORK, STEPHANIE J. MOELLER
• Format: Patent
• Language: Portuguese
• Published: 06.09.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

A method of detecting a biological activity, comprising: providing, a sample that may comprise a first biological activity; a first indicator reagent that can be converted by the first biological activity to a first biological derivative; a gelling agent; and a plurality of particles, each particle comprising an organic polymer that receives and retains the first biological derivative, wherein the particles have a mean particle diameter between 0.05 microns and 20 microns; forming a hydrogel comprising the first indicator reagent and the plurality of particles; wherein at least a portion of the plurality of particles is dispersed in the hydrogel or disposed on a major surface of the hydrogel; bringing the sample into fluid communication with a portion of the hydrogel; and detecting the presence or absence of the first biological derivative, wherein the first biological derivative is retained by the plurality of particles; wherein detecting the presence or absence of the first biological derivative comprises detecting a fluorescent first biological derivative or a colored first biological derivative; and wherein the presence of the first biological derivative is indicative of the presence of a first biological activity.