CULTURE MEDIUM FOR IN VITRO DIHAPLOIDIZATION OF HAPLOID TABACCO PLANTS

This culture medium finds application for a speedy creation of consolidated tobacco lines and varieties with high economic value. It reduces the time for obtaining dihaploide plants and ensures a high rate of dihaploidisation. The medium contains the following macroelements, in mg/l: 1,000-3,000 NH4...

Full description

Saved in:
Bibliographic Details
Main Authors SHVETS, STEPAN A, ENCHEVA, JULIJA KH, GEORGIEV, GEORGI J, KINTJA, PAVEL K, STOILOVA, MARIJA I
Format Patent
LanguageEnglish
Published 15.09.1993
Edition5
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:This culture medium finds application for a speedy creation of consolidated tobacco lines and varieties with high economic value. It reduces the time for obtaining dihaploide plants and ensures a high rate of dihaploidisation. The medium contains the following macroelements, in mg/l: 1,000-3,000 NH4NO3, 150-200 KNO3, 150-400 MgSO4.7H2O, 100-200 KH2PO4, 300-550 CaCl2.2H2O, 50-100 sodium ethylenediaminetetraacetate, 20-80 FeSO4.7H2O; microelements - 5.0-6.5 H3BO3, 20-30 MnSO4.4H2O, 0.5-1.0 ZnSO4.7H2O, 0.2-0.5 KJ, 0.02-0.1 Na2MoO4, 0.02-0.1 CuSO4.5H2O, 0.02-0.1 CoCl2.6H2O; vitamins - 80-120 myo-inosit, 0.3-0.6 PP, 0.3-0.6 B6, 0.1-0.3 B1, 20-50 g/l sucrose, 6-10 g/l agar, 0.1-0.3 indolylacetic acid, 0.1-0.3 6-benzyl aminopurine and 5-100 nicotianoside.
Bibliography:Application Number: BG19910094609