Tgf-beta fusion proteins and their use in wound healing

An artificial skin is prepared by impregnating a collagen matrix with a transforming growth factor- beta having a collagen-binding site to bind the growth factor to the collagen matrix, incubating the impregnated matrix with a source of fibroblasts and mesenchymal stem cells to form a captured popul...

Full description

Saved in:
Bibliographic Details
Main Authors TAI-LAN TUAN, DAVID T. CHEUNG, LINTAO WU, FREDERICK L HALL, MARCEL E. NIMNI
Format Patent
LanguageEnglish
Published 24.12.1996
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DISINFECTION, STERILISATION, OR DEODORISATION OF AIR
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES
MEDICAL OR VETERINARY SCIENCE
METALLURGY
METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:An artificial skin is prepared by impregnating a collagen matrix with a transforming growth factor- beta having a collagen-binding site to bind the growth factor to the collagen matrix, incubating the impregnated matrix with a source of fibroblasts and mesenchymal stem cells to form a captured population of mesenchymal stem cells within the impregnated matrix and incubating the resultant matrix with a source of keratinocytes which epithelialize the matrix to form an artificial skin. The collagen matrix is preferably in the form of a collagen sheet. The transforming growth factor- beta can be transforming growth factor- beta 1, transforming growth factor- beta 2 or transforming growth factor- beta 3. Preferably, the transforming growth factor- beta having a collagen binding site is a fusion protein having a purification tag, at least one proteinase site, an extracellular matrix binding site and a transforming growth factor active fragment. The extracellular matrix binding site binds collagen, fibronectin or a cell surface. A method of preparing the fusion protein involves purifying and renaturing transforming growth factor- beta protein to provide an active fusion protein.
Bibliography:Application Number: AU19960061529