Cell line comprising the promoter of cyclooxygenase-2 (cox-2) and a witness gene, and use thereof in the search of cox-2 transcriptional induction selective inhibitors
The cell lines comprises a DNA construct that comprises all or part of a promoter sequence of the gene coding for cyclooxygenase 2 (cox-2) and a reporter gene, operatively joined to each other, such that said promoter sequence of the cox-2 gene controls the expression of said reporter gene in respon...
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Main Authors | , |
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Format | Patent |
Language | English |
Published |
30.01.2001
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Edition | 7 |
Subjects | |
Online Access | Get full text |
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Summary: | The cell lines comprises a DNA construct that comprises all or part of a promoter sequence of the gene coding for cyclooxygenase 2 (cox-2) and a reporter gene, operatively joined to each other, such that said promoter sequence of the cox-2 gene controls the expression of said reporter gene in response to a suitable stimulus. The assay method comprises bringing said cell line into contact with the compound to be assayed and determining the existence of a signal indicative of the expression of activity due to the reporter gene. This method is suitable for the induction at a transcriptional level of cox-2 by suitable stimuli. |
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Bibliography: | Application Number: AU20000058289 |