ASSAY METHOD USING ENZYMES AS LABELLING SUBSTANCES

The present invention provides a method for determining a ligand or receptor which comprises carrying out an assay for the ligand or receptor, the assay requiring a labelled component, wherein the labelled component is a conjugate between a ligand or a receptor and a primary enzyme that is itself ca...

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Bibliographic Details
Main Author SELF, C.H
Format Patent
LanguageEnglish
Published 30.05.1985
Edition3
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:The present invention provides a method for determining a ligand or receptor which comprises carrying out an assay for the ligand or receptor, the assay requiring a labelled component, wherein the labelled component is a conjugate between a ligand or a receptor and a primary enzyme that is itself capable of producing or removing a modulator for a secondary system or that is the first enzyme in an enzyme system that is capable of producing or removing a modulator for a secondary system, and determining that portion of labelled component to be determined by allowing the primary enzyme and any other enzymes in the enzyme system, to produce or remove the modulator for the secundary system, allowing the secondary system to function in the presence or absence as appropriate of the modulator, and determining a product of the secondary system.
Bibliography:Application Number: AU19800063379