Process for the expression of genes of the dengue viruses

• Main Authors: ANABEL ALVAREZ ACOSTA, RAYNER RODRIGUEZ DIAZ, DELFINA ESPERANZA ROSARIO DOMINGUEZ, MAYRA MUNE JIMENEZ, VALEXYS VAZQUEZ VILLASUSO, CARLOS AUGUSTO SARIOL CURBELO, MARIA GUADALUPE GUZMAN TIRADO, GERARDO ENRIQUE GUILLEN NIETO, SUSANA VAZQUEZ RAMUDO, GABRIEL JESUS MARQUEZ PERERA, RAYSA VAZQUEZ CAMPOS, JOSE GARCIA SUAREZ
• Format: Patent
• Language: English
• Published: 22.06.1998
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to the field of biotechnology and to recombinant DNA techniques and particularly to a process for the expression of the gene which codes for the protein of the envelope of the dengue viruses serotypes 2 and 4 in methylotrophic yeasts of the genus Pichia. The objective is to obtain an immunogene for human vaccination. For the dengue serotype 2 virus, we started from the genic sequence which codes for the protein E of the virus, from the strain A 15 isolated during the dengue fever epidemic and haemorrhagic dengue which occurred in Cuba in 1981. In the case of the serotype 4 dengue virus, we started from the genic sequence which codes for the protein E of the virus from the strain 814669 isolated during the dengue fever epidemy which occurred in Dominica in 1981.