DNA CONSTRUCT AND MODIFIED YEAST

A DNA construct is described which comprises DNA encoding for a beta -galactosidase which can be secreted by a yeast, said DNA being flanked at its 5' end in reading frame by a yeast promoter sequence. Such DNA constructs can be incorporated in a plasmid vector (e.g. pVK11) and inserted into a...

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Bibliographic Details
Main Authors BRIAN SELBY HARTLEY, SUNDARAM RAMAKRISHNAN, VIJAY KUMAR
Format Patent
LanguageEnglish
Published 09.10.1990
Edition5
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
FODDER
FOODS OR FOODSTUFFS
HUMAN NECESSITIES
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
VINEGAR
WINE
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Summary:A DNA construct is described which comprises DNA encoding for a beta -galactosidase which can be secreted by a yeast, said DNA being flanked at its 5' end in reading frame by a yeast promoter sequence. Such DNA constructs can be incorporated in a plasmid vector (e.g. pVK11) and inserted into a suitable host, such as a yeast (e.g. Saccharomyces cerevisiae), to produce a microorganism which synthesises and secretes beta -galactosidase extracellularly or into the periplasmic space and which can grow on a substrate containing lactose, e.g. whey or whey permeate. The DNA coding for the beta -galactosidase may be of fungal origin, for example it may be derived from Aspergillus niger in the form of its lacA+ gene.
Bibliography:Application Number: AU19900052632