Sterilization of liquid chromatography resins

• Main Authors: ALOIS JUNGBAUER, HANS PETER LETTNER
• Format: Patent
• Language: English
• Published: 21.12.1995
• Edition: 6
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; PERFORMING OPERATIONS; PEST REPELLANTS OR ATTRACTANTS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; SEPARATION; TESTING; THEIR RELEVANT APPARATUS; TRANSPORTING; TRAPPING

A method for sterilizing liquid chromatography resins that are highly resistant to oxidation by strongly oxidizing agents and a sterilization solution for use therein. In a preferred embodiment, the invention comprises contacting an oxidant-resistant chromatographic resin with an aqueous solution of peracetic acid and up to 40% ethanol. In particular, where the solution is to be used to sterilize a positively charged ion-exchange resin, the solution preferably additionally includes an acetate buffer of a sufficient ionic strength to prevent adsorption of the peracetic acid ions on the resin.