Methods for regulating vascularization using gef containing nek-like kinase (gnk)

• Main Authors: C. DUKE VIRCA, JACQUES J. PESCHON, JOHN E. SIMS, CYNTHIA R. WILLIS, TIMOTHY A. BIRD
• Format: Patent
• Language: English
• Published: 03.07.2000
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention provides a novel use for GNK in treating pathological conditions related to angiogenesis. The present invention also provides isolated DNA encoding sGNK, expression vectors comprising the isolated DNA, and a method for producing sGNK by cultivating host cells containing the expression vectors under conditions appropriate for expression of the sGNK. Antibodies directed against sGNK or an immunogenic fragment thereof are also disclosed. The sGNK, which is a physiological substrate of GNK and co-purifies with GNK on gel filtration chromatography, may also be useful in treating vascularization abnormalities.