FLOW CELLS AND METHODS

An example of a flow cell includes a substrate having depressions separated by interstitial regions; a polymeric hydrogel positioned within each of the depressions; and a plurality of transposome complexes immobilized within each of the depressions by a biotin-containing linker. In this example, eac...

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Main Authors FRASER, Louise Jane, BASUKI, Johan Sebastian, ARTIOLI, Gianluca Andrea, DILL, Tyler J, MOORE, Jessica, CIESLA, Craig, HO, Yuan Heng Trevor, VON HATTEN, Xavier, RICOULT, Sebastien Georg Gabriel, MARTINS VITORIANO, Maria Ines, WEIR, Jacqueline C, GORMLEY, Niall Anthony, KARUNAKARAN, Aathavan, THOMSON, Vicki S
Format Patent
LanguageEnglish
Published 03.10.2024
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Summary:An example of a flow cell includes a substrate having depressions separated by interstitial regions; a polymeric hydrogel positioned within each of the depressions; and a plurality of transposome complexes immobilized within each of the depressions by a biotin-containing linker. In this example, each of the plurality of the transposome complexes is of a single type including a transposon end with a portion of a transferred strand hybridized to a portion of a non-transferred strand, wherein the transferred strand includes a first amplification domain and is free of an index sequence.
Bibliography:Application Number: AU20230396017