ALTERED CYTIDINE DEAMINASES AND METHODS OF USE

• Main Authors: RAVICHANDRAPRABHU, Lekha, NIRANTAR, Saurabh, BEH, Leslie Yee Ming, TAN, Shu Ting, TZENG, Kathleen, GHOMI, Hamed Tabatabaei, BUSBY, Kayla, GROSS, Stephen, SHULTZABERGER, Sarah E, BRUSTAD, Eric, FAHMI, Zahra, MATHONET, Pascale, TOH, Dewei Joel, BROWN, Colin, LAM, Huy, KARADEEMA, Rebekah, TRACZYK, Anna, YUNGHANS, Allison Kathleen
• Format: Patent
• Language: English
• Published: 04.01.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present disclosure is concerned with proteins, methods, compositions, and kits for mapping of methylation status of nucleic acids, including 5-methylcytosine and 5-hydroxymethyl cytosine (5hmC). In one embodiment, proteins are provided that selectively act on certain modified cytosines of target nucleic acids and converts them to thymidine or modified thymidine analogues. In another embodiment, proteins are provided that selectively act on certain modified cytosines of target nucleic acids and converts them to uracil or thymidine and selectively do not act on other certain modified cytosines of target nucleic acids. Also provided are compositions and kits that include one or more of the proteins and methods for using one or more of the proteins.