High amylose wheat - III

• Main Authors: LI, Zhongyi, JOBLING, Stephen Alan, LI, Xinguo, REGINA, Ahmed
• Format: Patent
• Language: English
• Published: 14.04.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The present invention provides wheat grain of the species Triticum aestivum, the grain comprising i) mutations in each of its SSIIa genes such that the grain is homozygous for a mutation in its SSIIa-A gene, homozygous for a mutation in its SSIIa-B gene and homozygous for a mutation in its SSIIa-D gene, wherein at least two of the mutations in said SSIIa genes are null mutations, ii) a total starch content comprising an amylose content and an amylopectin content, iii) a fructan content which is increased relative to wild-type wheat grain on a weight basis, preferably between 3% and 12% of the grain weight, iv) a -glucan content, v) an arabinoxylan content, vi) a cellulose content. The grain has a weight of between 25mg and 60mg, and the amylose content is between 45% and 70% on a weight basis of the total starch content of the grain as determined by iodine binding assay. The amylopectin content on a weight basis is reduced relative to the wild type wheat grain, and each of the -glucan content, arabinoxylan content and cellulose content are increased relative to the wild-type wheat grain on a weight basis, such that the sum of the fructan content, -glucan content, arabinoxylan content and cellulose content is between 15% and 30% of the grain weight.