Improvement of expression and folding in the manufacturing process of CD-RAP by using a CD-RAP precursor protein

• Main Authors: JANOCHA, Bernd, BARTNIK, Eckart, STOMMES, Judith, HABERMANN, Paul, SAAS, Joachim, SCHOETTLE, Isabell, STILLGER, Ursula, JESKE, Volker
• Format: Patent
• Language: English
• Published: 06.05.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Improvement of expression and folding in the manufacturing process of CD-RAP by using a CD-RAP precursor protein Abstract The present invention relates to a CD-RAP precursor protein comprising a pre-sequence and CD-RAP, and its use in manufacturing of native CD-RAP. The present invention further relates to a composition comprising CD-RAP and at least one positively charged amino acid and a buffer, pharmaceuticals comprising said composition, their use in methods of treating and/or preventing cartilage disease or injury in patients suffering from aggrecan degradation, and/or increased influx of water into the cartilage, and/or decreased CD-RAP expression, as well as methods of producing said composition and methods of storing CD RAP in said composition. The present invention further relates to a composition comprising liposomes comprising encapsulated CD-RAP or a variant thereof, its use in methods of treating joint disease or injury, and methods of producing such liposomal composition as well as storing CD-RAP therein.