Hepatoprotective and Antioxidant activity of a Novel active herbal agent against Non-alcoholic fatty liver disease

• Main Authors: Uddin, A. B. M. Helal, Yadav, Anil, Das, Suvadra, K., Hanumanthachar Joshi, Verma, Pritt, Chaubey, Madhvi
• Format: Patent
• Language: English
• Published: 14.04.2022
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

In the present invention we evaluated hepatoprotective and antioxidant activity of the ethanolic leaves extract of Abroma augusta on non-alcoholic fatty liver disease (NAFLD) in SD rats by different screening models. The experimental animals were divided in to five groups (n = 6) and then ethanolic leaves extract of Abroma augusta (ELEAA) in dose of 250 and 500 mg/kg body weight was administered. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), total cholesterol (TC) in the serum and high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), free fatty acid (FFA), Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) in liver tissue was evaluated respectively. While hepatoprotective effect were also confirmed by histopathological analysis, the expression level of tumor necrosis factor- a (TNF-a) and cytochrome P450 (CYP) 2E1 in Sprague - Dawley (SD) rats liver was determined by immunohistochemistry analysis. A significant decrease were observed in the level of serum ALT (2.85 fold), AST (2.17 fold), and the blood lipid TG (2.44 fold) and TC (1.76 fold) in the dose of 500mg/kg ethanolic leaves extract of Abroma augusta (ELEAA) treated Sprague Dawley (SD) rats (p < 0.01), compared to the standard groups.