Multiplex Detection of Magnaporthe oryzae and Sarocladium oryzae in Rice Seed using a Foldable Membrane Platform based on LAMP Technology

• Main Authors: Pramesh, Devanna, Parivallal, P Buela, Raj, Edwin, Mahesh, H. B, Prasanna Kumar, M. K
• Format: Patent
• Language: English
• Published: 12.08.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

Abstract The present disclosure relates to a method for developing LAMP-based foldable microdevice platform for detecting M.oryzae and S.oryzae in rice seed. The said method comprises the following steps: developing a LAMP, polymerase chain reaction (PCR), quantitative PCR (qPCR), and helicase dependent amplification (HDA) assays with primers, specifically targeting Magnaporthe oryzae (M. oryzae) and Sarocladium oryzae (S. oryzae) genome; comparing sensitivity of LAMP, HDA, PCR, and qPCR assays; and developing a portable foldable microdevice platform using optimized LAMP assay conditions to detect M. oryzae and S. oryzae in rice seeds, wherein said platform serves as a prototype for developing on field diagnostic kits to be used at a point of care centers for rapid diagnosis of M. oryzae and S. oryzae in rice seeds. m "I CL E _ -0 WUr C:m0j cm 0 tEO.LL (U (U 0- C oJC EEoo2 2 :E C Q,0 m .2 CL