Method for detecting different beta-casein variants in cow milk

• Main Authors: Gao, Yundong, Wei, Xiaochao, Jiang, Qiang, Wang, Lingling, Zhang, Yaran, Gao, Yaping, Ju, Zhihua, Zhong, Jifeng, Wang, Xiuge, Huang, Jinming
• Format: Patent
• Language: English
• Published: 22.04.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

Abstract The present invention provides a method for detecting different p-casein variants in cow milk. The method comprises: Si. extracting a protein from a sample to be tested; S2. enzymatically hydrolyzing the extracted protein by using trypsin and a V8 protease; S3. detecting the peptide fragments obtained from the enzymatically hydrolyzing of the protein by mass spectrometry to identify the p-casein variants. The detection method of the present invention has a reasonable design, can effectively detect different p-casein variants (Al, A2, A3, B, C, E, F, HI, I) in a dairy product, and has high detection accuracy and simple operations. The present invention can determine an A2 type p-casein variant of the terminal dairy product of dairy cows, and can regulate the A2 milk dairy product market. The present invention is a good application of protein detection technology in production practice, and can produce huge economic and social benefits.