Purification of oligosaccharides from a fermentation broth by using filtration

• Main Authors: KRAHN, Jan Henrik, HELFRICH, Markus, JENNEWEIN, Stefan
• Format: Patent
• Language: English
• Published: 06.01.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; SPIRITS; SUGARS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

A method for the purification of an oligosaccharide, preferably a neutral human milk oligosaccharide (HMO) or a sialylated HMO, from a fermentation broth, the method comprising: - providing a fermentation broth which contains the oligosaccharide of interest, biomass, microbial cells and carbohydrates other than the oligosaccharide of interest; - removing the microbial cells from the fermentation broth, thereby providing a process stream; - subjecting the process stream to a first filtration step using a nanofiltration membrane, thereby providing a filtrate which contains the oligosaccharide of interest; - subjecting the filtrate to a second filtration step using a nanofiltration membrane, thereby providing a retentate which contains the oligosaccharide of interest; and - removing salts from the process stream using electrodialysis thereby providing a purified preparation of the oligosaccharide of interest.