Cell culture carrier, and method and device for producing same

• Main Authors: ARATANI, Tomoyuki, IWASHITA, Natsuko, YAMAZAKI, Takehiro, SHIONOIRI, Momoko, SAMESHIMA, Tatsuya, YAGINUMA, Hidekazu, SATOH, Naoki
• Format: Patent
• Language: English
• Published: 14.10.2021
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OF MACROMOLECULAR COMPOUNDS; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

A method for producing a cell culture carrier configured from a hydrogel having high shape retentivity and a device for producing the cell culture carrier are developed, and a structure comprising a cell culture carrier produced using the method and device is provided.　The method has: a holding step for causing a first solution to be held on a support, the first solution including a multibranched polymer that has polyethylene glycol as the skeleton and has one functional group from among one or more nucleophilic functional groups or electrophilic functional groups in a side chain and/or at an end; and a gel formation step for causing a second solution to land, using a droplet discharge device, so as to contact the first solution held on the support to form one or more dot-shaped hydrogels, the second solution containing a multibranched polymer that has polyethylene glycol as the skeleton and has the other functional group from among the one or more nucleophilic functional groups or electrophilic functional groups in a side chain and/or at an end.