Double fluorescent quantitative RT-PCR detection method for Classical swine fever virus and Bovine viral diarrhea virus

• Main Authors: Zhang, Yue, Chen, Jing, Zhang, Hexiao, Tian, Fulin, Sun, Shengfu, Tian, Ye, Wei, Xuehua, Li, Yujie, Wang, Guisheng, Chen, Shumin
• Format: Patent
• Language: English
• Published: 25.02.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Abstract Disclosed relates to a double fluorescent quantitative RT-PCR detection method for a Classical swine fever virus and a Bovine viral diarrhea virus, and belongs to the technical field of fluorescent quantitative RT-PCR. The detection method uses two sets of primers and probes; the sequence of an amplification product of the first set of primers and probe is uniquely consistent with a conserved region of a Classical swine fever virus gene sequence, and the sequence of an amplification product of the second set of primers and probe is uniquely consistent with a conserved region of a BVDV gene sequence; in addition, the CSFV probe P only appears in the amplification product consistent with the conserved region of the Classical swine fever virus gene sequence, and the BVDV probe P only appears in the amplification product consistent with the conserved region of the Bovine viral diarrhea virus gene sequence (each set of primers and probe can eliminate the interference of the other set of primers and probe). Therefore, the detection method can distinguish CSFV and BVDV, and can simultaneously detect the Classical swine fever virus and the Bovine viral diarrhea virus. (Fig. 1) Drawings 0 7m Fig.1I kin INf acra Fig. 2