Method For Preparation Of Solid Phase Extraction Column Of Sophocarpine Molecularly Imprinted Polymer

• Main Authors: GESANGZHUOGA, SILANGYUZHEN, BAN, Dan, MA, Xingbin, JI, Peng, LABACIDAN, HE, Bingmei, YONG, Yanhong, WUJINCUOMU, LIN, Hongling
• Format: Patent
• Language: English
• Published: 20.08.2020
• Subjects: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; THEIR PREPARATION OR CHEMICAL WORKING-UP; THEIR RELEVANT APPARATUS; TRANSPORTING

Abstract The present invention discloses a method for preparation of solid phase extraction column of sophocarpine molecularly imprinted polymer, including precipitation polymerization method for preparation of dual-template imprinted polymer. Dissolve the template (OSC, 0.04 mmol) in 5 mL of chloroform, refrigerate it at 4 °C for 30 min, add cross-linking agent (ethylene glycol dimethacrylate, 1 mmol), initiator (ABN, 0.15 mmol) and ACN (25 mL) and degas the solution with N2 for 15 min, then place the solution in 60 °C water bath for 24 h, at 150 rpm. After polymerization, screen the polymer particles in ACN, and retain the solid polymer particles with 22 m nylon membrane. In order to remove the template, extract the polymer particles by Soxhlet extraction until OSC can't be detected by HPLC-MS/MS. Clean the residues in d-MIPs with ACN and water. d-NIPs are prepared in the same way without template. Drawings 15.OkV 16.0mm x50.0k 11/5/2017 21:02 1.00un 60 -EXI3OSC - [---sc 0) E 5o 0) o 30 - 101 0 (, MeOH ACN Water loading solvent