Method for screening molecular marker of cattle adapting to high altitude hypoxia and application thereof

• Main Authors: WANG, Xiuge, JIANG, Qiang, ZHANG, Yaran, HUANG, Jinming, GAO, Yaping, LIU, Wenhao, ZHAO, Han, JU, Zhihua, LIU, Yong, WANG, Lingling, WANG, Jinpeng, WEI, Xiaochao, GAO, Yundong
• Format: Patent
• Language: English
• Published: 23.02.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Provided are a method for screening a specific molecular marker of cattle adapting to high altitude hypoxia, and use of the specific marker in screening cattle breeds or individuals suitable for survival in high altitude hypoxia. For the genetic characteristics of adaptation of cattle breeds to high altitude hypoxia, from the perspective of genome evolution, selection and adaptation, local cattle breeds distributed at high and low altitudes are selected, and multi-genome-selection-signal and genome-wide association analysis methods and strategies are integrated to screen a key gene and a molecular marker adapting to high altitude hypoxia. The detection method designed according to the key gene and the marker features high accuracy and easy operation during application. By the analysis of cattle breeds at different altitudes, gene ACSS2 related to adaptation to high altitude hypoxia and a haplotype thereof are found, and specific SNP that receives the strongest selection signal on the gene is located. The method has great significance and practical value for cattle molecule breeding.