Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde

Abstract Method, composition, kit and system for isolating amplifiable nucleic acid from specimens preserved in a liquid-based cytology preservative that contains formaldehyde. The technique relies on the use of 2-imidazolidone and a protease enzyme, such as proteinase K, at elevated temperatures. A...

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Bibliographic Details
Main Authors WILSON, Timothy Joseph, JENSEN, Deborah Christine, KIRKCONNELL, Brett Wolfe
Format Patent
LanguageEnglish
Published 23.01.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
DERIVATIVES THEREOF
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
SPIRITS
SUGARS
VINEGAR
WINE
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Summary:Abstract Method, composition, kit and system for isolating amplifiable nucleic acid from specimens preserved in a liquid-based cytology preservative that contains formaldehyde. The technique relies on the use of 2-imidazolidone and a protease enzyme, such as proteinase K, at elevated temperatures. Advantageously, RNA can be isolated and used as a template in nucleic acid amplification reactions. 11890699_1 (GHMatters) P104017.AU.2
Bibliography:Application Number: AU20190284088