A nucleic acid probe, a method of immobilizing the nucleic acid to a solid support using UV light, a solid support comprising an immobilized nucleic acid probes, and a test device comprising a solid support

• Main Authors: CHIN, Wai Hoe, HELLER, Martin, ERIKSEN, Johan, JENSEN SØE, Martin
• Format: Patent
• Language: English
• Published: 27.02.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

Disclosed are nucleic acid probes, a method of immobilizing the nucleic acid to a solid support, a solid support comprising an immobilized nucleic acid probes, and a test device comprising a solid support. The nucleic acid probe includes a terminus anchor chain portion, and a capture portion wherein the terminus anchor chain portion includes a sequence of at least 18 nucleotides composed of stretches of up to 5 nucleotides of base type X with intermediate nucleotide(s) of base type Cytosine (C) and optionally one nucleotide of base type Guanine (G) or a sequence with at least 90 % similarity thereto, wherein each base type X independently of each other designate base type Thymine (T) or base type Uracil (U). The nucleic acid probe is immobilized to the solid support using UV light. Further disclosed is a test system comprising a solid support having at least one supercritical angle fluorescence structure (SAF structure).