Mutated protoporphyrinogen IX oxidase (PPX) genes

• Main Authors: De Schopke, Aura, Gocal, Gregory F.W, Beetham, Peter R, Dumm, Sarah, Pearce, James, Walker, Keith A, Schopke, Christian
• Format: Patent
• Language: English
• Published: 27.02.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

Provided are compositions and methods relating to gene and/or protein mutations in transgenic or non-transgenic plants. In certain embodiments, the disclosure relates to mutations in the protoporphyrinogen IX (PPX) gene. In some embodiments the disclosure relates to plants that 5 are herbicide resitant. Figure 1: Amino acid sequence of Arahidopsis thaliana chloroplast protoporphyrinogen oxidase (P POX - At4g01690) (Accession # AX084732) (SEQ ID NO: 1) 1 MELSLLRPTTQSLLPSFSKPNLRLNVYKPLRLRCSVAGGPTVGSSKIEGG2 51 cGTTITTDCVIVGGG7SGLC-AQALATKHPDAAPNLIVTEAKDRVGGNII 5 101 TiE NGFLWEEGPNSFQPSDPMLTMVVDSGLKDDLVLGDPTAPRFVLWNG 151 KLRPVPSKLTDLPFFDLMSIGCKIRACFGALG:RPSPPGREESVEEFVRR 201 NLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNGGSIIGGTF 251 KAIQERKNAPKAERDPRLPKPQGQTVGSFR KGLRMLPEAISARLGSKVKL 301 SWTLSGITKLESGGYNLTYETPDGLVSVQSKSVVMTVPSHVASGLLRPLS 10 351 ESAAIAJLSKLYYPPV/AAVSISYPKEAIRTECLIDGELKGFGQLHPRTQGV 401 ETLGTIYSSSLFPNRAPPGRILLLNYTGGSTNTGILSKSEGELVEAVDRD 451 LRKMLIKPNSTDPLKLVRVWPQAIPQFLVGHFDILDTAKSSLTSSGYEG 501 LFLGGNYVAGVALGRCVEGAYETAIENNFMSRYAYK 15 Figure 2: Nucleotide sequence of Arabidopsisrhaliana chloroplastprotoporphyrinogen oxidase (PPOX - At4g01690) (Accession # AX084732) (SEQ ID NO: 2) 1 TGACAAAATT CCGAATTCTC TGCGATTTCC ATGGAGTTAT CTCTTCTCCG TCCGACGACT 61 CAATCGCTTC TTCCGTCGTT TTCGAAGCCC AATCCCGAT TAAATGTTTA TAAGCCTCTT 20 121 AGACTCCGTT GTTCAGTGGC CGGTGGACCA ACCGTCGGAT CTTCAAAAAT CGAAGGCGGA 181 GGAGGCACCA CCATCACGAC GGATTGTGTG ATTGTCGGCG GAGGTATTAG TGGTCTTTGC 241 ATCGCTCAGG CGCTTGCTAC TAAGCATCCT GATGCTGCTC CGAATTTAAT TGTGACCGAC 301 GCTAAGGATC GTITTGGAGG CAACATTATC ACTCGTGAAG AGAATGGTTT TCTCTGGGAA 361 GAAGGTCCCA ATAGTTTTCA ACCGTCTGAT CCTATGCTCA CTATGGTGGT AGATAGTGGT 25 421 TTGAAGGATG ATTTGGTCTT GGGAGATCCT ACTGCGCCAA GGTTTGTGTT GTGGAATGGG 481 AAATTGAGGC CGGTTCCATC GAAGCTAACA GACTTACCGT TCTTTGATTT GATGAGTATT 541 GGTGGGAGA TTAGAGCTGG TTTTGGTGCA CTTGGCATTC GACCGTCACC TCCAGTCGT 601 GAAGAATCTG TGGAGGAGTT TGTACGGCGT AACCTCGGTG ATGAGGTTTT TGAGCGCCTG 661 ATTGAACCGT TTTGTTCAGG TGTTTATGCT GGTGATCCTT CAAAACTGAG CATGPAAGCA 30 721 GCGTTTGGSA AGGTTTGGAA ACTAGAGCAA AATGGTGGAA GCATAATAGG TGGTACTTTT 781 AAGGCAATTC AGGAGAGGAA AAACGCTCCC AAGGCAGAAC GAGACCCGCG CCTGCCAAAA 841 CCACAGGGCC AAACAGTTGG TTCTTTCAGG AAGGGACTTC GAATGTTGCC AGAAGCAATA 901 TCTGCAAGAT TAGGTAGCAA AGTTAAGTTG TCTTGGAASC TCTCAGGTAT CACTAAGCTG 961 GAGAGCGGAG GATACAACTT AACATATGAG ACTCCAGATG GTTTAGTTTC CGTGCAGAGC 35 1021 AAAACTGTTG TAATGACGGT GCCATCTCAT GTGCAAGTG GTCTCTTGCG CCCTCTTTCT 1081 GAATCTGCTG CAAATGCACT CTCAAAACTA TATTACCCAC CAGTTGCAGC ACTATCTATC 1141 TCGTACCCGA AAGAAGCAAT CCGAACAGAA TGTTTGATAG ATGGTGAACT AAAGGGTTTT 1201 GGGCAATTGC ATCCACGCAC GCAAGGAGTT GAAACATTAG GAACTATCTA CAGCTCCTCA 1261 CTCTTTCCAA ATCGCGCACC GCCCGGAAGA ATTTTGCTGT TGAACTACAT TGGCGGGTCT 40 1321 ACAAACACCG GAATTCTGTC CAAGTCTGAA GGTGAGTTAG TGGAAGCAGT TGACAGAGAT 1381 TTGAGGAAAA TGCTAATTAA GCCTAATTCG ACCGATCCAC TTAAATTAGG AGTTAGGGTA 1441 TGGCCTCAAG CCATTCCTCA GTTTCTAGTT GGTCACTTTG ATATCCTTGA CACGGCTAAA 1501 TCATCTCTAA CGTCTTCGGG CTACGAAGGG CTATTTTTGG GTGGCATTA CGTCGCTGGT 1561 GTAGCCTTAG GCCGGTGTGT AGAAGGCGCA TATGAAACCG CGATTGAGGT CAACAACTTC 45 1621 ATGTCACGGT ACGCTTACAA GTAAATGTAA AACATTAAAT CTCCCAGCTT GCGTGAGTTT 1681 TATTAAATAT TTTGAGATAT CCAAAAAAAA AAAAAAA Figure 3: Amine acid sequence ofArabidopsis thaliana mitochondrial protoporphyrinogen oxidase (At5g14220 Accession #NM 121426) (SEQ ID NO: 3) 50 1 iASGAVADIvASGKRVAVVGAGJVSKGL-AA YKSRGiTVJEADGR 51 VGGKLRSVMNGLIWDEGANTMTEEEVGSLLDLGLREKQQFPISQKK