Method of nucleic acid fragmentation

The present invention provides a method of preparing an at least partially randomly sheared library of nucleic acids, said method including providing a source of nucleic acids, randomly incorporating modified bases into the nucleic acids, and digesting the modified 5 nucleic acids with one or more m...

Full description

Saved in:
Bibliographic Details
Main Authors Shinozuka, Hiroshi, Cogan, Noel Oliver Ian
Format Patent
LanguageEnglish
Published 06.09.2018
Subjects
CHEMISTRY
COMBINATORIAL CHEMISTRY
LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES
METALLURGY
Online AccessGet full text

Cover

More Information
Summary:The present invention provides a method of preparing an at least partially randomly sheared library of nucleic acids, said method including providing a source of nucleic acids, randomly incorporating modified bases into the nucleic acids, and digesting the modified 5 nucleic acids with one or more modification-dependent restriction endonucleases to produce the nucleic acid library. The present invention also provides kits for performing the method, nucleic acid libraries prepared by the method and methods of next generation sequencing using the method.
Bibliography:Application Number: AU20140308567