Method of nucleic acid fragmentation
The present invention provides a method of preparing an at least partially randomly sheared library of nucleic acids, said method including providing a source of nucleic acids, randomly incorporating modified bases into the nucleic acids, and digesting the modified 5 nucleic acids with one or more m...
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Main Authors | , |
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Format | Patent |
Language | English |
Published |
06.09.2018
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Subjects | |
Online Access | Get full text |
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Summary: | The present invention provides a method of preparing an at least partially randomly sheared library of nucleic acids, said method including providing a source of nucleic acids, randomly incorporating modified bases into the nucleic acids, and digesting the modified 5 nucleic acids with one or more modification-dependent restriction endonucleases to produce the nucleic acid library. The present invention also provides kits for performing the method, nucleic acid libraries prepared by the method and methods of next generation sequencing using the method. |
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Bibliography: | Application Number: AU20140308567 |