Alpha-amylase variants

A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 4, 6, 13, 1...

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Bibliographic Details
Main Authors ABBAS, HANNA ELISABET, JONG, DE RENE MARCEL, RIJ, VAN EVERT TJEERD
Format Patent
LanguageEnglish
Published 22.10.2015
Subjects
ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES
BAKERY PRODUCTS
BAKING
BEER
BIOCHEMISTRY
CANDLES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DETERGENT COMPOSITIONS
DETERGENTS
EDIBLE DOUGHS
ENZYMES
ENZYMOLOGY
FATTY ACIDS THEREFROM
HUMAN NECESSITIES
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PRESERVATION THEREOF
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
RECOVERY OF GLYCEROL
RESIN SOAPS
SOAP OR SOAP-MAKING
SPIRITS
TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS
USE OF SINGLE SUBSTANCES AS DETERGENTS
VINEGAR
WINE
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Summary:A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 4, 6, 13, 14, 15, 16, 20, 45, 47, 51, 54, 61, 68, 69, 70, 71, 72, 73, 74, 75, 77, 78, 80, 82, 87, 88, 94, 95, 100, 103, 104, 117, 124, 125, 126, 128, 130, 133, 134, 136, 143, 144, 146, 168, 174, 177, 178, 183, 186, 188, 189, 190, 194, 195, 199, 200, 201, 204, 207, 210, 214, 217, 219, 222, 225, 227, 233, 234, 235, 236, 240, 251, 252, 254, 258, 259, 260, 261, 262, 263, 264, 266, 267, 269, 271, 273, 281, 282, 283, 284, 286, 288, 299, 322, 323, 325, 327, 328, 331, 334, 350, 356, 358, 367, 370, 371, 374, 377, 378, 388, 391, 414, 421, 422, 445, 450, 467, 488, 505, 536, 548, 554, 583, 588, 603, 637, 648, 651, 652, 660, 676, 677, said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having alpha-amylase activity. Such a variant polypeptide may be used in the preparation of a baked product.
Bibliography:Application Number: AU20140222636