MODIFIED BIOTIN-BINDING PROTEIN

Disclosed is a modified biotin-conjugated protein which is characterized by having any one substitution selected from the group consisting of items 1) to 6) shown below in a protein which comprises the amino acid sequence depicted in SEQ ID NO:2 or a mutant sequence thereof and has a biotin binding...

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Bibliographic Details
Main Authors TSUNASHIMA, MASAKO, SOFUKU, KOZUE, TAKAKURA, YOSHIMITSU
Format Patent
LanguageEnglish
Published 23.07.2015
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Disclosed is a modified biotin-conjugated protein which is characterized by having any one substitution selected from the group consisting of items 1) to 6) shown below in a protein which comprises the amino acid sequence depicted in SEQ ID NO:2 or a mutant sequence thereof and has a biotin binding activity: 1) the substitution of a serine residue at position-36 in SEQ ID NO:2 for an amino acid residue incapable of forming a hydrogen bond; 2) the substitution of a tryptophan residue at position-80 in SEQ ID NO:2 for a hydrophilic amino acid residue; 3) the substitution of an aspartic acid residue at position-116 in SEQ ID NO:2 for an amino acid residue incapable of forming a hydrogen bond; 4) the substitution of a proline residue at position-46 in SEQ ID NO:2 for a threonine, serine or tyrosine residue, and the substitution of a threonine residue at position-78 in SEQ ID NO:2 for an amino acid residue incapable of forming a hydrogen bond; 5) the substitution of a proline residue at position-46 in SEQ ID NO:2 for a threonine, serine or tyrosine residue, and the substitution of an aspartic acid residue at position-116 in SEQ ID NO:2 for an amino acid residue incapable of forming a hydrogen bond; and 6) the substitution of a proline residue at position-46 in SEQ ID NO:2 for a threonine, serine or tyrosine residue, the substitution of a threonine residue at position-78 in SEQ ID NO:2 for an amino acid residue incapable of forming a hydrogen bond, and the substitution of an aspartic acid residue at position-116 in SEQ ID NO:2 for an amino acid residue incapable of forming a hydrogen bond.
Bibliography:Application Number: AU20090348580