Quantification of nucleic acid molecules using multiplex PCR
Described are novel quantification methods and systems that permit, within the context of multiplex PCR, the quantification of all targets within a single reaction tube. The methods employ quantitation algorithms applied to the amplification profiles of internal calibration controls or standards uti...
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Main Authors | , , , , , , |
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Format | Patent |
Language | English |
Published |
05.09.2013
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Subjects | |
Online Access | Get full text |
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Summary: | Described are novel quantification methods and systems that permit, within the context of multiplex PCR, the quantification of all targets within a single reaction tube. The methods employ quantitation algorithms applied to the amplification profiles of internal calibration controls or standards utilizing a plurality of nucleic acid templates that are amplified within the same reaction tube as the nucleic acid target(s) interrogated. |
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Bibliography: | Application Number: AU20080323926 |