Quantification of nucleic acid molecules using multiplex PCR

Described are novel quantification methods and systems that permit, within the context of multiplex PCR, the quantification of all targets within a single reaction tube. The methods employ quantitation algorithms applied to the amplification profiles of internal calibration controls or standards uti...

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Bibliographic Details
Main Authors DANCIK, VLADIMIR, BRISTOL, MICHAEL, GARCIA, ELIZABETH, HART, KYLE, SLEPNEV, VLADIMIR, UNGER, JOHN, WELLS, PATRICK
Format Patent
LanguageEnglish
Published 05.09.2013
Subjects
CALCULATING
COMPUTING
COUNTING
ELECTRIC DIGITAL DATA PROCESSING
PHYSICS
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Summary:Described are novel quantification methods and systems that permit, within the context of multiplex PCR, the quantification of all targets within a single reaction tube. The methods employ quantitation algorithms applied to the amplification profiles of internal calibration controls or standards utilizing a plurality of nucleic acid templates that are amplified within the same reaction tube as the nucleic acid target(s) interrogated.
Bibliography:Application Number: AU20080323926