Method for generating stable cell lines expressing high levels of a protein of interest
This invention relates to industrial production of proteins. More specifically, the invention relates to a method for obtaining cells that stably express a protein of interest, even when cultivated in the absence of selective pressure. DHFR is used as a surrogate marker. The transfected cells are no...
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Main Authors | , |
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Format | Patent |
Language | English |
Published |
22.01.2009
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Subjects | |
Online Access | Get full text |
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Summary: | This invention relates to industrial production of proteins. More specifically, the invention relates to a method for obtaining cells that stably express a protein of interest, even when cultivated in the absence of selective pressure. DHFR is used as a surrogate marker. The transfected cells are not selected based on resistance to a toxic compound, but based on fluorescence as measured by FACS using fluorescent MTX. |
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Bibliography: | Application Number: AU20080277632 |