Method for introducing biologically active molecules into the nucleus of eukaryotic cells using electric current
Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors, each connected to a high voltage source. Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other bio...
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Main Authors | , , , , , , , , , , |
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Format | Patent |
Language | English |
Published |
28.10.2010
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Subjects | |
Online Access | Get full text |
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Summary: | Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors, each connected to a high voltage source. Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors (7, 8), each connected to a high voltage source (5, 6). Each capacitor discharges via a power transistor (9, 10) into a cuvette holding the cell sample. The first capacitor is charged with a preset voltage U 1 and the second with a voltage U 2 given by the equation: U 2 = R x I 2 x K 2, R 1the resistance of the cuvette and suspension in it I 2the desired current K 2a correction factor based on the properties of thee cuvette ACTIVITY : None given. MECHANISM OF ACTION : Gene therapy. No supporting data provided. |
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Bibliography: | Application Number: AU20070202279 |