Method for introducing biologically active molecules into the nucleus of eukaryotic cells using electric current

Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors, each connected to a high voltage source. Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other bio...

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Main Authors CORINNA THIEL, HERBERT MULLER-HARTMANN, LUDGER ALTROGGE, KIRSTEN ROTHMANN, REGOR SIEBENKOTTEN, MEIKE WEIGEL, HEIKE WESSENDORF, ELKE LORBACH, GUDULA RIEMEN, RAINE CHRISTINE, JULIANA HELFRICH
Format Patent
LanguageEnglish
Published 28.10.2010
Subjects
APPARATUS FOR CONVERSION BETWEEN AC AND AC, BETWEEN AC AND DC,OR BETWEEN DC AND DC, AND FOR USE WITH MAINS OR SIMILAR POWERSUPPLY SYSTEMS
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BASIC ELECTRONIC CIRCUITRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CONTROL OR REGULATION THEREOF
CONVERSION OF DC OR AC INPUT POWER INTO SURGE OUTPUTPOWER
CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
CULTURE MEDIA
ELECTRICITY
ELECTROTHERAPY
ENZYMOLOGY
GENERATION
HUMAN NECESSITIES
HYGIENE
MAGNETOTHERAPY
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
PULSE TECHNIQUE
RADIATION THERAPY
SPIRITS
ULTRASOUND THERAPY
VINEGAR
WINE
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Summary:Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors, each connected to a high voltage source. Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors (7, 8), each connected to a high voltage source (5, 6). Each capacitor discharges via a power transistor (9, 10) into a cuvette holding the cell sample. The first capacitor is charged with a preset voltage U 1 and the second with a voltage U 2 given by the equation: U 2 = R x I 2 x K 2, R 1the resistance of the cuvette and suspension in it I 2the desired current K 2a correction factor based on the properties of thee cuvette ACTIVITY : None given. MECHANISM OF ACTION : Gene therapy. No supporting data provided.
Bibliography:Application Number: AU20070202279