Variants of Hepatitis B virus resistant against some nucleoside analogues, but sensitive to others, and uses thereof

• Main Authors: KAISER, ROLF, SCHILDGEN, OLIVER, ROCKSTROH, JUERGEN, VOGEL, MARTIN, DAEUMER, MARTIN, MATZ, BERTFRIED, WEITNER, LUTWIN, GERLICH, WOLFRAM, SCHEWE, CARL KNUD, HELM, MARTIN
• Format: Patent
• Language: English
• Published: 16.08.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates generally to the field of Hepatitis B variants exhibiting a reduced sensitivity to nucleoside analogues both and . More in particular, reverse transcriptase mutant rt 1233 V is provided. Present invention provides assays and methods for detecting such variant, which assays are useful in monitoring anti- viral therapeutic regimes and adjusting patient therapy. A diagnostic kit for detecting the presence of an HBV variant in a biological sample has also been described. Finally, the use of a farmaceutical composition to cure a subject suffering from a HBV infection, which HBV is resistant to lamuvidine and/or adefovir has been provided, which farmaceutical composition comprises the nucleoside analogue tenofovir.