Methods for prediction of clinical outcome to epidermal growth factor receptor inhibitors by cancer patients

• Main Authors: MARILEILA VARELLA GARCIA, PAUL A JR. BUNN, WILBUR A. FRANKLIN, FEDERICO CAPPUZZO, FRED R. HIRSCH
• Format: Patent
• Language: English
• Published: 15.12.2005
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a method to obtain an expression score to evaluate gene expression in a tissue specimen obtained from a person having or suspected of having cancer comprising: a) determining by immunohistochemistry a fraction score, wherein the fraction score corresponds to the percentage of cells in the tissue specimen that stain positive for a biomarker; b) determining an intensity score that corresponds to the staining intensity of the biomarker in the sample of tumor cells; c) calculating an expression score that is a numerical evaluation of the product of the fraction score and the intensity score.