Genes encoding proteolytic enzymes from aspargilli

• Main Authors: WOLFRAM KEMMNER, ULRIKE FOLKERS, CHRISTIAN WAGNER, SABINE KLUGBAUER, RICHARD ALBANG, SYLVIA HOPPER, PHILIPP KRUBASIK, DIETER MAIER, ERIK KIMPEL, PAMELA TAN, OLIVER HEINRICH, KAJ ALBERMANN, JOSEPHINE STIEBLER, ANDREAS FRITZ, ALEX STOCK, FABIO SPREAFICO, WILK GUSTEDT VON, LUPPO EDENS, ALBERTUS, ALARD DIJK VAN
• Format: Patent
• Language: English
• Published: 01.03.2007
• Subjects: AGEING; BEER; BIOCHEMISTRY; CHEMISTRY; CLARIFICATION; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PASTEURISATION; PEPTIDES; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS; PHYSICS; PRESERVATION; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; PROTEIN COMPOSITIONS FOR FOODSTUFFS; PURIFICATION; SPIRITS; STERILISATION; TESTING; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE; WORKING-UP PROTEINS FOR FOODSTUFFS

The invention relates to newly identified gene sequences that encode novel proteases obtainable from Aspergillus niger. The invention features the full length gene sequence of the novel genes, their cDNA sequences as well as the full-length functional protein and fragments thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with DNA according to the invention and cells wherein a protease according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.