VERFAHREN ZUR HERSTELLUNG VON PLASMID DNS IN PHARMAZEUTISCHER QUALITÄT
This invention provides a process for the continuous alkaline lysis of a bacterial suspension in order to harvest pDNA. It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatogragphy, and hydrophobic intera...
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Main Authors | , , , , |
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Format | Patent |
Language | German |
Published |
15.02.2011
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Subjects | |
Online Access | Get full text |
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Summary: | This invention provides a process for the continuous alkaline lysis of a bacterial suspension in order to harvest pDNA. It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatogragphy, and hydrophobic interaction chromatography. These optional purification steps can be combined with the continuous lysis in order to produce a highly purified pDNA product substantially free of gDNA, RNA, protein, endotoxin, and other contaminants. |
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Bibliography: | Application Number: AT20050739527T |