HOCHDURCHSATZ- BIOMOLEKULARE- KRISTALLISATION UND SCREENING VON BIOMOLEKULAREN KRISTALLEN

• Main Authors: ELLSON, RICHARD, N, STEARNS, RICHARD, G, MUTZ, MITCHELL, W
• Format: Patent
• Language: German
• Published: 15.08.2006
• Edition: 7
• Subjects: AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUSPOLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR; APPLYING LIQUIDS OR OTHER FLUENT MATERIALS TO SURFACES, IN GENERAL; ATOMISING APPARATUS; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; CORRECTION OF TYPOGRAPHICAL ERRORS; CRYSTAL GROWTH; GENERAL METHODS OF ORGANIC CHEMISTRY; i.e. MECHANISMS PRINTING OTHERWISE THAN FROM A FORME; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; LINING MACHINES; METALLURGY; NOZZLES; ORGANIC CHEMISTRY; PEPTIDES; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PRINTING; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITHDEFINED STRUCTURE; REFINING BY ZONE-MELTING OF MATERIAL; SELECTIVE PRINTING MECHANISMS; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITHDEFINED STRUCTURE; SINGLE-CRYSTAL-GROWTH; SPRAYING APPARATUS; SPRAYING OR ATOMISING IN GENERAL; STAMPS; THEIR RELEVANT APPARATUS; TRANSPORTING; TYPEWRITERS; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL ORUNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL

The present invention provides a method for the acoustic ejection of fluid droplets from fluid-containing reservoirs to form small volumes high throughput combinatorial experimentation for crystallization. The method is especially suited to preparing combinatorial libraries of small volume crystallization experiments for crystallizing difficult to crystallize biomacromolecules. The small volumes conserve costly and difficult to obtain macromolecules and permit an increased number of experimental crystallization conditions tested for an amount of the biomacromolecule of interest for crystallization. The time required for the experiments is greatly reduced by the scaled down experimental volumes. The invention is conducive to forming high density microarrays of small volume crystallization experiments. Acoustic detection of crystals in situ and distinction between biomacromolecular and non-biomacromolecular crystals is also taught.