TRANSGENE PFLANZEN MIT VERBESSERTER TOLERANZ GEGEN HERBIZIDE DER PHOSPHOMETHYLGLYCINFAMILIE, DIE EIN FÜR MUTIERTE 5-ENOLPYRUVYLSHIKIMATE-3- PHOSPHATE SYNTHASE KODIERENDES GEN ENTHALTEN

• Main Authors: SAILLAND, ALAIN, LEBRUN, MICHEL, FREYSSINET, GEORGES
• Format: Patent
• Language: German
• Published: 15.04.2006
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

New gene (I) encodes 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS) that contains at least the Thr102Ile mutation. Also new are: (1) EPSPS mutant protein (II) with this mutation; (2) chimeric gene contg. (I) plus heterologous 5' and 3' regulatory elements; (3) vectors for plant transformation contg. at least one chimeric gene as in (2); and (4) plant cells (and plants derived from them) contg. the chimeric gene. The gene is pref. of bacterial or plant origin, in particular it is from Salmonella typhimurium or maize. The gene may further comprise at least one additional mutation, esp. resulting in the substitution Pro106Ser or Gly101Ala. The chimeric gene includes a promoter from a plant virus or plant, e.g. alpha -tubulin, histone, etc. Opt. it also includes a sequence encoding a transit peptide.