ALKALINE PROTEASE, VERFAHREN ZU IHRER HERSTELLUNG,ANWENDUNG DERSELBEN UND DIESE HERSTELLENDE MIKROORGANISMEN

• Main Authors: YONEDA, TADASHI, FUKUYAMA, SHIRO, MIYOTA, YOSHIAKI
• Format: Patent
• Language: German
• Published: 15.11.2004
• Edition: 7
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; BEER; BIOCHEMISTRY; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENT COMPOSITIONS; DETERGENTS; ENZYMOLOGY; FATTY ACIDS THEREFROM; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF GLYCEROL; RESIN SOAPS; SOAP OR SOAP-MAKING; SPIRITS; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

A new strain (deposit No. FERM BP-5736) of Bacillus sp., a protease highly stable in surfactants with the following properties obtained by cultivation of the strain or its mutants, its manufacturing method, and use of the protease. (1) hydrolyzes proteins or peptides, (2) the optimal pH of around 12 for incubation with casein as a substrate at 30 DEG C for 24 hours, (3) the stable pH range of 5 to 11 for incubation with casein as a substrate at 30 DEG C for 24 hours, (4) the optimal temperature of around 60 DEG C for incubation with casein as a substrate at pH 10 for 10 minutes, (5) the molecular weight of 29,000+/-2,000 by SDS-polyacrylamide gel electrophoresis, (6) the isoelectric point of 10.1+/-0.5 by isoelectric focusing polyacrylamid gel electrophoresis, and (7) the residual activity of not less than 60% at 40 DEG C at 30 minutes after mixing with a 500 ppm LAS solution and not less than 20% at 55 DEG C after 15 minutes.