SELEKTIONMARKER-GENFREIE REKOMBINANTE STÄMME: VERFAHREN ZUR IHRER HERSTELLUNG UND DIE VERWENDUNG DIESER STÄMME

The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesire...

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Bibliographic Details
Main Authors SWINKELS, BART WILLEM, VAN GORCOM, ROBERTUS FRANCISCUS MARIA, SELTEN, GERARDUS CORNELIS MARIA
Format Patent
LanguageGerman
Published 15.05.2003
Edition7
Subjects
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Summary:The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
Bibliography:Application Number: AT19940201896T