Rat Placental Lactogen II Gene: Characterization of Gene Structure and Placental-Specific Expression1
Rat placental lactogen II (rPLII) was the first described member of the rat PRL-like placental gene family in which nine novel proteins have now been identified. In this article, we present data on the isolation and characterization of the rPLII gene. Two genomic clones, GC I (18.5 kb) and GC II (9....
Saved in:
Published in | Endocrinology (Philadelphia) Vol. 139; no. 3; pp. 967 - 973 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Endocrine Society
01.03.1998
|
Online Access | Get full text |
Cover
Loading…
Abstract | Rat placental lactogen II (rPLII) was the first described member of the
rat PRL-like placental gene family in which nine novel proteins have
now been identified. In this article, we present data on the isolation
and characterization of the rPLII gene. Two genomic clones, GC I (18.5
kb) and GC II (9.4 kb), were isolated from an EMBL3 Sprague-Dawley rat
liver genomic DNA library. GC I, which was used for further analysis,
contains the entire coding region and extensive 5′ and 3′ flanking
information. The rPLII gene, estimated to be 5.4 kb in size, has the
same five-exon and four-intron structure and identical intron/exon
splice sites and types as the rPRL gene. A major transcription start
site 58 bp upstream of the initiator methionine codon and several minor
sites 1–3 bp 5′ and 3′ of this site were identified by primer
extension of day 18 placental messenger RNA. The rPLII gene has been
localized to chromosome 17, using a series of hybrid cell lines derived
from mouse hepatoma cells (MWTG3) and adult rat hepatocytes; this is
the same chromosome designation as the PRL gene itself and other cloned
placental members of this gene family. Luciferase reporter constructs
containing 5′ flanking DNA sequences were tested in transient
transfection assays in the rat choriocarcinoma cell line, Rcho, and the
rat pituitary GC cell line. Both a 4.5- and 3-kb 5′ flanking sequence
supported luciferase expression in the Rcho but not the GC cells. A
765-bp fragment showed no activity in either cell type. Transient
transgenic mice, generated with the 3-kb 5′ rPLII/luciferase construct,
expressed varying amounts of luciferase expression in the placenta. |
---|---|
AbstractList | Rat placental lactogen II (rPLII) was the first described member of the
rat PRL-like placental gene family in which nine novel proteins have
now been identified. In this article, we present data on the isolation
and characterization of the rPLII gene. Two genomic clones, GC I (18.5
kb) and GC II (9.4 kb), were isolated from an EMBL3 Sprague-Dawley rat
liver genomic DNA library. GC I, which was used for further analysis,
contains the entire coding region and extensive 5′ and 3′ flanking
information. The rPLII gene, estimated to be 5.4 kb in size, has the
same five-exon and four-intron structure and identical intron/exon
splice sites and types as the rPRL gene. A major transcription start
site 58 bp upstream of the initiator methionine codon and several minor
sites 1–3 bp 5′ and 3′ of this site were identified by primer
extension of day 18 placental messenger RNA. The rPLII gene has been
localized to chromosome 17, using a series of hybrid cell lines derived
from mouse hepatoma cells (MWTG3) and adult rat hepatocytes; this is
the same chromosome designation as the PRL gene itself and other cloned
placental members of this gene family. Luciferase reporter constructs
containing 5′ flanking DNA sequences were tested in transient
transfection assays in the rat choriocarcinoma cell line, Rcho, and the
rat pituitary GC cell line. Both a 4.5- and 3-kb 5′ flanking sequence
supported luciferase expression in the Rcho but not the GC cells. A
765-bp fragment showed no activity in either cell type. Transient
transgenic mice, generated with the 3-kb 5′ rPLII/luciferase construct,
expressed varying amounts of luciferase expression in the placenta. |
Author | Shah, Paresh Duckworth, Mary Lynn Sun, Yuxiang Szpirer, Claude |
Author_xml | – sequence: 1 givenname: Paresh surname: Shah fullname: Shah, Paresh – sequence: 2 givenname: Yuxiang surname: Sun fullname: Sun, Yuxiang – sequence: 3 givenname: Claude surname: Szpirer fullname: Szpirer, Claude – sequence: 4 givenname: Mary Lynn surname: Duckworth fullname: Duckworth, Mary Lynn |
BookMark | eNqdj0FLAzEQhQep4FY9e80f2DWz2bqt11K10EOx3kNIZzVlmZQkC-KvN1sKvfc0zLz3hvdNYcKeCeAJZYU1ymfiva9QLSpVzeZqfgMFLppZ2WIrJ1BIiaps67q9g2mMh7w2TaMKoE-TxLY3ljiZXmyMTf6bWKzX4p2YXsXyx4R8pOD-THKehe9OitilMNg0BBKG95cX5e5I1nXOitXvMVCMOYMPcNuZPtLjed7Dy9vqa_lRjqVtcEwnqz74IXA2aJR6hNKjrjOUVnqEUlcH_wH4AVvT |
ContentType | Journal Article |
DOI | 10.1210/endo.139.3.5838 |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Anatomy & Physiology |
EISSN | 1945-7170 |
EndPage | 973 |
ExternalDocumentID | 10_1210_endo_139_3_5838 |
GroupedDBID | - 08R 0R 1TH 29G 2WC 34G 39C 3O- 3V. 4.4 48X 53G 55 5GY 5RE 5RS 5YH 79B 7X7 88E 8F7 8FI 8FJ 8RP AABJS AABMN AAIMJ AAJQQ AAPBV AAPQZ AAUQX AAYJJ ABEFU ABFLS ABPPZ ABSAR ABUFD ABUWG ACGFS ACIMA ACIWK ACPRK ACUTJ ADACO ADBBV ADBIT ADEIU ADGZP ADHKW ADIYS ADRTK AELNO AELWJ AEMDU AENEX AENZO AETBJ AETEA AEWNT AFDAS AFFNX AFKRA AFRAH AFXEN AGINJ AGKRT AGVJH AHMBA AIKOY AIMBJ ALMA_UNASSIGNED_HOLDINGS APIBT AQKUS ARIXL ASMCH AYOIW AZQFJ BAWUL BAYMD BBAFP BCRHZ BENPR BEYMZ BGYMP BHONS BPHCQ BSWAC BTRTY BVXVI C1A CDBKE CJ0 CS3 DAKXR DIK DPPUQ DU5 DZ E3Z EBS EJD ENERS F5P FA8 FH7 FHSFR FOTVD FQBLK FYUFA GAUVT GJ GJXCC GX1 H13 HZ H~9 IAO IH2 IHR ITC J5H KBUDW KOP KQ8 KSN L7B M1P M5 MBLQV MHKGH MVM NLBLG NOYVH NVLIB O0- O9- OBH ODMLO OHM OHT OK1 OVD P2P PQEST PQQKQ PQUKI PRINS PROAC PSQYO Q-A REU RIG ROX ROZ TCN TJX TLC TMA TWZ UPT VQP W2D WH7 WOQ X X52 X7M XJT XZ YQI ZA5 ZCG ZGI ZKB ZXP ZY1 |
ID | FETCH-endocrinepress_journals_10_1210_endo_139_3_58383 |
ISSN | 0013-7227 |
IngestDate | Tue Jan 05 21:44:21 EST 2021 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-endocrinepress_journals_10_1210_endo_139_3_58383 |
ParticipantIDs | endocrinepress_journals_10_1210_endo_139_3_5838 |
PublicationCentury | 1900 |
PublicationDate | 1998-03 |
PublicationDateYYYYMMDD | 1998-03-01 |
PublicationDate_xml | – month: 03 year: 1998 text: 1998-03 |
PublicationDecade | 1990 |
PublicationTitle | Endocrinology (Philadelphia) |
PublicationYear | 1998 |
Publisher | Endocrine Society |
Publisher_xml | – name: Endocrine Society |
SSID | ssj0014443 |
Score | 3.1645048 |
Snippet | Rat placental lactogen II (rPLII) was the first described member of the
rat PRL-like placental gene family in which nine novel proteins have
now been... |
SourceID | endocrinepress |
SourceType | Publisher |
StartPage | 967 |
Title | Rat Placental Lactogen II Gene: Characterization of Gene Structure and Placental-Specific Expression1 |
URI | http://dx.doi.org/10.1210/endo.139.3.5838 |
Volume | 139 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1ZS8NAEF5qBVFQtFW82QfxpSS2Js3hW6lK44V4QH0KabKxBU0KNmD7f_yfzmw227RVsL6EsplOmp2vszuzcxByZILGY1UrVEJYfMBAMX3F0pimGB0TzZHQDni5pts7o_WsX7Xr7ULhKxe1lAw6qj_6Ma_kP1KFMZArZsnOIVnJFAbgM8gXriBhuP5Jxg9g3d-jH5ynNN5g6xygrTgOryaNxn5T1mMeyb0h3sPT6GR8eiCZKLwffdjzsQZyGiIb1Sa891EQg6JJvfG4PUWPDJaa7Hcx7Fa6FR67nog8BC7S5fyYcCX3knwCKl_l6KjfE121m29eEkisyf7VIqtoWLkZRtHYUZFl7uUcFdnvY1k8al4t1zTFPE2LBKgs1cS2XlfA1qxOqOq08JHApJZTvLZh5tZwO22PMrM8gH3LgRLEKrBSNRUPjccroYxPRMsIaF2kdIHS1VykXCCLWG8RWzScO9fysErXRXCmeAtRQQqTo6YetUJWWTYNXIi5vczTOlkTRghtpIjaIAUWlUi5EXmD-H1IjykPC-YSLpGlWxF9USYM8EYlVGiGN-o4FDF1RqfRRuOQ36ESbRTQRmfRRnNo2yTG5cVTs6VMvoMr_hEf7i_Tpm2RYhRHbJtQ3WKWFxp2UA0tPfCZx0zP8wMrCE0bW6PtkJM5me_O_Y09sjyG5z4pwgywA9hZDjqHXK7fPiCB-g |
link.rule.ids | 314,780,784,27924,27925 |
linkProvider | Flying Publisher |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Rat+Placental+Lactogen+II+Gene%3A+Characterization+of+Gene+Structure+and+Placental-Specific+Expression1&rft.jtitle=Endocrinology+%28Philadelphia%29&rft.au=Shah%2C+Paresh&rft.au=Sun%2C+Yuxiang&rft.au=Szpirer%2C+Claude&rft.au=Duckworth%2C+Mary+Lynn&rft.date=1998-03-01&rft.pub=Endocrine+Society&rft.issn=0013-7227&rft.eissn=1945-7170&rft.volume=139&rft.issue=3&rft.spage=967&rft.epage=973&rft_id=info:doi/10.1210%2Fendo.139.3.5838&rft.externalDocID=10_1210_endo_139_3_5838 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0013-7227&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0013-7227&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0013-7227&client=summon |