Rat Placental Lactogen II Gene: Characterization of Gene Structure and Placental-Specific Expression1
Rat placental lactogen II (rPLII) was the first described member of the rat PRL-like placental gene family in which nine novel proteins have now been identified. In this article, we present data on the isolation and characterization of the rPLII gene. Two genomic clones, GC I (18.5 kb) and GC II (9....
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Published in | Endocrinology (Philadelphia) Vol. 139; no. 3; pp. 967 - 973 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Endocrine Society
01.03.1998
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Online Access | Get full text |
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Summary: | Rat placental lactogen II (rPLII) was the first described member of the
rat PRL-like placental gene family in which nine novel proteins have
now been identified. In this article, we present data on the isolation
and characterization of the rPLII gene. Two genomic clones, GC I (18.5
kb) and GC II (9.4 kb), were isolated from an EMBL3 Sprague-Dawley rat
liver genomic DNA library. GC I, which was used for further analysis,
contains the entire coding region and extensive 5′ and 3′ flanking
information. The rPLII gene, estimated to be 5.4 kb in size, has the
same five-exon and four-intron structure and identical intron/exon
splice sites and types as the rPRL gene. A major transcription start
site 58 bp upstream of the initiator methionine codon and several minor
sites 1–3 bp 5′ and 3′ of this site were identified by primer
extension of day 18 placental messenger RNA. The rPLII gene has been
localized to chromosome 17, using a series of hybrid cell lines derived
from mouse hepatoma cells (MWTG3) and adult rat hepatocytes; this is
the same chromosome designation as the PRL gene itself and other cloned
placental members of this gene family. Luciferase reporter constructs
containing 5′ flanking DNA sequences were tested in transient
transfection assays in the rat choriocarcinoma cell line, Rcho, and the
rat pituitary GC cell line. Both a 4.5- and 3-kb 5′ flanking sequence
supported luciferase expression in the Rcho but not the GC cells. A
765-bp fragment showed no activity in either cell type. Transient
transgenic mice, generated with the 3-kb 5′ rPLII/luciferase construct,
expressed varying amounts of luciferase expression in the placenta. |
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ISSN: | 0013-7227 1945-7170 |
DOI: | 10.1210/endo.139.3.5838 |